Tuesday, May 1, 2012: 2:00 PM
Waterbury Ballroom, 2nd fl (Sheraton New Orleans)
Linda Lehmann
1, Ausra Peciulyte
2, Tomas Larsson
3, Stuart M. Stocks
4, Henrik Steen Jorgensen
4, Timothy Hobley
1 and
Lisbeth Olsson5, (1)Center for Microbial Biotechnology, Technical University of Denmark, Kgs. Lyngby, Denmark, (2)Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden, (3)Innventia, Stockholm, Sweden, (4)Fermentation Pilot Plant, Novozymes A/S, DK-2880 Bagsvaerd, Denmark, (5)Industrial Biotechnology, Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
Trichoderma reesei is well recognised for its potential in producing cellulolytic enzymes and has been used extensively for industrial production. However, the linkage between the cultivation process conditions, the amounts and types of cellulolytic and other lignocellulose degrading enzymes produced, and in turn, how hydrolysis will subsequently be affected is poorly understood. In our work we have focused on establishing this relationship.
The cellulolytic hyper-producing and glucose de-repressed strain T. reesei Rut-C30 was used in batch fermentations using cellulose, both model cellulose substrate and cellulose resulting from alkaline cooking, as carbon source. The influence of pH and temperature on the enzyme production profile was also investigated. The total extracellular protein, filter paper- and endoglucanase-activity were determined. Further analysis of the enzyme mixtures produced was performed using SDS-PAGE and LC-MS-MS in order to understand the reasons for difference in enzyme performance.
The crude enzyme preparations (fermentation broth) were used for evaluation of its hydrolysis performance using either cellulose model substrates, alkaline cooked spruce or pre-treated corn stover. The importance of the cellulose structural properties for its degree of hydrolysis was investigated.
