9-35: Fermentative production of L-Lysine-L-Lactate from fractional green juices from a green biorefinery

Tuesday, May 1, 2012
Napoleon Ballroom C-D, 3rd fl (Sheraton New Orleans)
Birgit Kamm, Research Institute Biopos e.V. and BTU Cottbus, Teltow, Germany, Sebastian Leiss, Research Institute Bioactive Polymer Systems e.V., Teltow, Germany and Joachim Venus, Bioengineering, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim, Potsdam, Germany
For a future biobased economy, lactic acid is an important starting substance for industrial important intermediate products like poly lactic acid or acrylic acid [1]. Aminium lactates have been shown to be a good feedstock for the synthesis of dilactide without the formation of byproducts. They can be produced in a lactic acid fermentation by using aqueous solutions of organic amines as pH-correction solution. L-lysine-L-lactate was produced by using 50% L-lysine for pH-correction and different fractions of green juice as fermentation medium. Green juice contains carbohydrates, proteins, free amino acids and inorganic salts which are essential for microbial growth and can therefore be used as fermentation medium for lactic acid bacteria. In a second refining step these compounds should be separated. To find out in which way the separation of proteins affects the fermentation, non-treated juice and deproteinized juice were compared with MRS-medium.  The trials show that the fermentative production of L-lysine-L-lactate can be integrated into the system Green Biorefinery, where deproteinized press juice accrues as a product [2].

[1]        B. Kamm, P.R. Gruber, M. Kamm, Biorefineries - Industrial Processes and Products, Wiley-VCH 2006.

[2]       S. Leiß, J. Venus, B. Kamm, Fermentative Production of L-Lysine-L-lactate with Fractionated Press Juices from the Green Biorefinery. Chem. Eng. Technol. 33, 2102-2105 (2010).

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