8-70: Ammonia soaking pretreatment for sweet sorgham under different conditions

Sweet sorghum was milled and sieved to 0.5-1.0 mm particle size using a Wiley mill. Two different ammonia soaking pretreatment conditions i.e. 15% NH₄OH at 60 ⁰C and 50% NH₄OH at 30 ⁰C for different sample to ammonium hydroxide ratio were evaluated with shaking at 150 rpm. Accellerase® 1500 was used to hydrolyze ammonia pretreated sweet sorghum samples. The results showed that 50 % NH₄OH at 30⁰C for sweet sorgham samples was not efficient to achieve sugar yield when compared to 15% NH₄OH (1:10) at 60⁰C.