Paper: Kinetics and mechanism of enzymatic hydrolysis of nanocrystalline cellulose (33rd Symposium on Biotechnology for Fuels and Chemicals (May 2-5, 2011))

Tuesday, May 3, 2011

Xiaohui Ju and Xiao Zhang, School of Chemical Engineering and Bioengineering, Center for Bioproducts and Bioenergy, Washington State University, Richland, WA

Decrystallization of cellulose is a major bottleneck in enzymatic hydrolysis of lignocellulosic biomass. The kinetic and mechanism of enzyme degradation of nanocrystalline cellulose (NCC) prepared from bleached softwood kraft pulp was investigated. The hydrolysability of three cellulase preparations obtained from Novozymes, Danisco and DSM on NCC were determined respectively. Cellulase hydrolysis of nanocrystalline cellulose has a much lower V_m compared with typical lignocelluloses substrates. We have also developed a new XRD procedure to illustrate the mechanism of enzyme decrystallization of NCC.

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