The promoter of glyceraldehyde-3-phosphate dehydrogenase gene (gpd) cloned from P. sordida YK-624 was used to drive expression of lignin peroxidase (LiP) gene from P. sordida YK-624, and the expression vector was transformed into a P. sordida YK-624. A transformant A-11, which produced approximately 5-folds LiP activity that wild type, was selected. This transformant showed higher ligninolytic activity and selectively than wild type in beech wood meal after 4-week incubation. Moreover, high glucose conversion was observed in the enzymatic saccharification of the wood meal which was treated with A-11, compared with that of the wild type-treated wood meal. These results suggest that high expression of LiP gene in P. sordida YK-624 improves both ligninolytic ability and saccharification of the fungal treated-wood meal.
We identified most produced protein of P. sordida YK-624 under solid ligninolytic condition, and cloned the gene (bee2) promoter. The promoter was used to drive the expression of manganese peroxidase gene, and the expression plasmid was constructed and transformed. All of these transformants showed higher ligninolytic activity and selectively than A-11 and wild type in beech wood meals after 4-week incubation. These results suggest that the bee2 promoter is useful regulator for high expression of genes under solid ligninolytic condition.