Paper: Understanding the differences between utilization of Avicel and pretreated lignocellulose by <i>Clostridium thermocellum</i> (33rd Symposium on Biotechnology for Fuels and Chemicals (May 2-5, 2011))

Tuesday, May 3, 2011

Xiongjun Shao¹, Mingjie Jin², Anna Guseva¹, Daniel Olson¹, Venkatesh Balan², Chaogang Liu³, David A. Hogsett³, Bruce Dale² and Lee Lynd¹, (1)Thayer School of Engineering at Dartmouth College, Hanover, NH, (2)Deparment of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI, (3)Mascoma Corporation, Lebanon, NH

Clostridium thermocellum is a model microorganism for consolidated bioprocessing (CBP) of lignocellulosic biomass to fuels and chemicals. One phenomenon observed for this organism is its much faster utilization of Avicel than its utilization of various pretreated lignocellulose. In this study, we report that initial catalyst loading affects final cellulose conversion for simultaneous saccharification and fermentation but not for C. thermocellum. Hydrolysis of Avicel and ammonia fiber expansion (AFEX) pretreated corn stover with C. thermocellum and isolated C. thermocellum cellulase indicate that the difference in final cellulose conversion between the two substrates is greater with whole cells than cellulase. Tests on hemicellulose removal and particle size reduction for AFEX pretreated corn stover indicate that substrate accessibility is very important for enhanced conversion by C. thermocellum. We will also report hydrolysis behavior of flowthrough pretreated poplar, comparing its conversion performance to Avicel.

See more of: Poster Session 2
See more of: General Submissions

<< Previous Paper | Next Paper >>

1-75: Understanding the differences between utilization of Avicel and pretreated lignocellulose by Clostridium thermocellum