Monday, April 19, 2010
LL Conference Facility (Hilton Clearwater Beach)
Agaricus brasiliensis CS1, Pleurotus ostreatus and Aspergillus flavus produced holocellulase when grown in solid and liquid cultures containing agro-industrial residue, including sugar cane bagasse and dirty cotton residue, as substrates. Those isolates proved to be an efficient producer of holocellulase under the conditions selected for this screening. The bromatological analysis of agro-industrial residues showed some differences, especially for protein, fiber, hemicelluloses, cellulose and lignin contents. The highest holocellulase activities (xylanase, mannanase, polygalacturonase, endoglucanase, FPAse and avicelase) were obtained after solid state cultivation at 10% of substrate concentration. In this case, remarkably levels of xylanase and pectinase activities were produced by A. flavus, followed by P. ostreatus. A. brasiliensis CS1 showed the best yield of reducing sugar production when grown on medium containing sugar cane bagasse. It was also the most efficient producer of protein, except when cultivated on dirty cotton residue, which had A. flavus as the best producer. The enzymatic hydrolysis of sugar cane bagasse and dirty cotton residue by crude extract samples of A. brasiliensis CS1, P. ostreatus and A. flavus showed that the best yield of reducing sugar production was achieved with sugar cane bagasse as the substrate.
Keywords: Agaricus brasiliensis · Agro-industrial residue · holocellulose · holocellulase · xylanase · pectinase
Supported by CNPq and FAPDF
Keywords: Agaricus brasiliensis · Agro-industrial residue · holocellulose · holocellulase · xylanase · pectinase
Supported by CNPq and FAPDF