Monday, April 19, 2010
12-33

Experimental design 23 to evaluate the ethanol production by Klebsiella pneumonia

Jouciane de Sousa Silva1, Jocélia de Sousa Mendes2, Maria Valderez Ponte Rocha3, Regiane Silva Pinheiro1, Giovanilton Ferreira Silva1, and Andrea Lopes de Oliveira Ferreira4. (1) Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, Fortaleza, Brazil, (2) Departamento de Engenharia Química, Unversidade Federal do Ceará, Campus do Pici, Bloco 709, Fortaleza, Brazil, (3) Chemical Engineering, Universidade Federal Rural do Semi-Árido, BR 110 - Km 47 Bairro Pres. Costa e Silva, Mossoró, Brazil, (4) Chemical Engineering, Universidade Federal do Ceará, Campus do Pici, Bloco 709, Fortaleza, Brazil

Biofuel production is growing and highlighting increasingly on the world market, due to production be originated from the renewable sources. Currently, it is growing demand for alternative sources for production of biofuel. One of the main and most promising sources is the use of biomass, which it is also increasing the use of industrial waste for this purpose. Currently an industrial waste that is becoming a problem for the biodiesel industry is glycerol, due to their generation as inevitable byproduct of the reaction of biodiesel production. Every 10 kg of biodiesel produced by transesterification of vegetable oils or animal fats, are generated 1 kg of crude glycerol. This glycerol increasing in the market caused a fall in its price, therefore, glycerol has become ideal for the production of compounds reduced through anaerobic digestion, i.e., it is an optimum carbon source. The aim of this study was evaluating the better condition of production of alcohol by fermentation using glycerol as carbon source by Klebsiella pneumoniae. For the evaluation of bioethanol production, it was carried, a 23 full factorial design. The variables studied were concentration of glycerol, temperature and concentration of sodium nitrate. Combined effects of temperature, glycerol and sodium nitrate concentration were investigated and optimized using response surface methodology. Optimum conditions for the production of bioethanol were mild temperature 40 ºC, glycerol concentration 20 g/L.

See more of Poster Session One Sponsored by Genencor
See more of General Submissions

See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)