Thursday, April 22, 2010 - 8:30 AM
9-02
Validating reliable feedstock supply
A 700 dry tonne corn stover pile was constructed in 2005, part of a $2 Million USDA funded project to investigate one-pass corn harvest and wet stover storage using the Ritter Method:
Circulating water thru the pile during construction, removing solubles while compacting and ensiling the material at 4.5 pH as the sucrose in the stover, ferments to organic acids
Annual storage samples evaluated by LORRE, Purdue University showed stover in the open storage pile, 30 m high with a 45 Year 2005 2006 2007 2008 Incoming Stover Annual Storage Samples % Dry Matter Glucan 31% 42% 42% 37% Holocellulose 59% 68% 66% 57% Acid Insoluble Residue 12% 17% 20% 22% Acid Soluble Lignin 3.8% 1.5% 1.8% 2.1% Ash 6.0% 6.7% 6.9% 6.9% Solubles 15% 4.8% 5.5% 8.1% Structural mass 84% 94% 95.2% 86% Dry Matter Balance 99% 98% 101% 94% The holocellulose yield was 94% and removing the solubles increased the stover holocellose fraction from 59% to 67% during the first two years of storage. In the third year, the pile height was diminished to 15 meters, exposing more surface area, incurring more holocellulose loss. The fermentation yield was 95% of theoretical yield, further validating the Ritter Method for feedstock storage up to two years. Stover hydrolysis was done at 4% and 15% solids loadings (w/v) and 15 FPU cellulase / g glucan. The hydrolyzate was fermented using xylose co-fermenting yeast. Final ethanol concentration was 34 g/L (3.4% wt/vol) after 48 hr.
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