Monday, April 19, 2010
3-12
Mining Dictyoglomus turgidum for enzymatically active carbohydrases
Dictyoglomus species represent a novel group of thermophilic, anaerobic organisms with considerable biotechnological promise; these organisms are so unique that they have been given their own genus, Dictoglomi. Dictyoglomus turgidum is an anaerobic, thermophile able to degrade a wide range of biomass components including starch, cellulose, pectin and lignin. The broad range of substrate utilization is reflected in the high percentage of CAZymes present in the genome, 2.35% of its total genes, higher than the percentage present in the cellulose-degrading, thermophilc anaerobe, Clostridium thermocellum, with 2.2% of its total genes. This apparent strong degradation capacity, coupled with the uniqueness of the organism, suggested that D. turgidum would be an excellent source of new and novel biomass-degrading enzymes. Screening of a random clone library generated from D. turgidum resulted in the discovery of several novel biomass-degrading enzymes with low homology to known molecules. Whole genome sequencing of the organism followed by bioinformatics-directed amplification of selected genes resulted in the recovery of additional novel enzyme molecules.
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