Monday, April 19, 2010
3-43
Evaluation of production of β-glucosidase, xylanase, β-xylosidase, feruil-esterase by Aspergillus awamori
There is an increasing demand to establish renewable feedstocks for chemical and fuel production that can decrease the dependency on dwindling oil reserves and reduce greenhouse gas emissions. Enzymatic hydrolysis of the biomass is essential for generation of fermentable sugars which are then converted to ethanol and involves the use of cellulases, hemicellulases and a host of ancillary enzymes. Aspergillus awamori is well known as an excellent producer of β-glucosidase. The objective of this study was to evaluate the production of β-glucosidase, xylanase, β-xylosidase, feruil-esterase by this fungus using different nitrogen sources. Initial results showed that wheat bran was the best carbon source for production of enzymes. The microorganism was cultivated in 30ºC/200 rpm in a culture medium with 30 g/L of wheat bran as carbon source and yeast extract, sodium nitrate, urea or ammonium sulfate were used as nitrogen sources in the adequate concentration maintaining the same relation carbon/nitrogen (C/N=10). The β-glucosidase production using yeast extract (12 IU/mL) was on average three times higher compared with other nitrogen sources tested. For xylanase production, the highest levels (50 IU/mL) were obtained with sodium nitrate, followed by medium with urea (36 IU / mL). The production of β-xylosidase achieved maximum levels with urea (0.7 IU/mL) or sodium nitrate (0.65 IU/mL). The feruil esterase production was higher in media containing ammonium sulphate (0.18 IU/mL) or urea (0.17 IU/mL). The results suggest the urea as ideal nitrogen source for the joint production of xylanase, β-xylosidase and feruil esterase by A. awamori.
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)