Monday, April 19, 2010
5-13
Small-scale conversion screen to identify sorghum with superior traits
Tanya Kruse, Anthy Alexiades, Greg East, Bonnie R. Hames, and Joon-Hyun Park. Ceres, Inc., 1535 Rancho Conejo Blvd, Thousand Oaks, CA 91320
In the development of improved energy crop varieties, obtaining accurate chemical composition and conversion performance characteristics will be critical. A small laboratory-scale, high-throughput assay has been developed that can be used to assess the relative conversion efficiency of sorghum biomass samples. Acidic pretreatment methods have been employed, and the assay is reproducible at the milligram scale. Ceres has available hundreds of sorghum samples, and the biomass collected from a subset of these lines has been assessed for relative digestibility in Ceres’ small-scale conversion assays. Lines with distinct differences in sugar released per gram dry biomass and/or in percent of theoretical sugar yields can be identified. This conversion assay provides a direct means to identify sorghum lines with superior conversion characteristics, leading to higher final sugar yields. This information will be invaluable to breeders and genetic engineers in the design of improved energy crop varieties.
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)
See more of General Submissions
See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)