Monday, April 19, 2010
3-60

Microbial fructosyltransferase: Production and characterization

Rafael F Perna1, Elda S Silva1, Gisella M. Zanin1, Flavio F Moraes1, Maria F.A. Rodrigues1, and Alfredo E. Maiorano2. (1) Chemical Engineering Department, State University of Maringa, Av. Colombo, 5790, BL D-90, Maringa, PR, 87020-900, Brazil, (2) Laboratory of Industrial Biotechnology, Institute for Technological Research of São Paulo State, Av. Prof. Almeida Prado, 532, São Paulo, 05508-901, Brazil

Fructosyltransferases (FTase, E.C. 2.4.19) are enzymes that catalyze the biotransformation of sucrose into fructooligosaccharides (FOS), which can be used as prebiotics. FOS can be extracted from plants or synthesized by microbial enzymes. In terms of industrial production, the use of microbial enzymes become interesting, especially those produced by Aspergillus sp. and Aerobasidium sp. The aim of the present work is to study the production of FTase and the effects of pH and temperature on the fructosyltransferase activity. The enzyme was produced by Aspergillus oryzae IPT 301 in a 10 liters bioreactor with growth medium containing sucrose as main carbon source. The test was performed at 800 rpm, 30 °C, 0.75 vvm and pH 4.5. The fructosyltransferase (At) and hydrolytic (Ah) activities were determined at temperatures ranging from 35 to 65 °C and pH from 3.5 to 6.5. It was observed that intracellular At increases with temperature, holding the maximum value at 55 - 65 °C, while the optimum pH value was 5.5. Optimum temperature for extracellular At ranged from 55 to 65 °C and optimum pH from 5.0 to 6.0. Furthermore, the optimum temperature for the intracellular Ah was 65 °C and optimum pH was 4.5 - 5.0. For extracellular Ah the optimum temperature and pH were 55 to 65 °C and 3.5 to 6.0, respectively.

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