Monday, April 19, 2010
3-57
Optimization of enzymatic hydrolysis of cassava starch for production of glucose syrup
Abstract: Enzymatic hydrolysis of starch for producing glucose syrups was evaluated at laboratory scale. Enzymes provided from Novozymes and Genecor International were compared. Alpha-amylase from Bacillus licheniformis (Liquozyme® SC DS from Novozymes), in a range of pH 5.7 - 6.0, temperature 82-86 ° C, enzyme dosage % (w/w) 0.013-0.025; and also glucoamylase of the Aspergillus niger (Spirizyme ® Fuel of Novozymes), in a range of pH of 3.5-5.5 , temperature 32-70 ° C, enzyme dosage % (w/w) 0.04-0.06; and finally the enzyme mixture of fungal a-amylase and Aspergillus Kawachi glucoamylase of Aspergillus niger (STARGEN 001 of International Genecor), in a range of 3.0-4.5 pH, fermentation temperature of 20-40 ° C, enzyme dosage of 1.0-2.5 kg / MT of dry powder were evaluated. The 3.5-dinitrosalicylic acid (DNS), glucose oxidase and Bradford analytical methods were used to measure and determine dextrose equivalent (ED) and enzyme activity, glucose concentration and protein concentration, respectively. Additionally, standardized and validated conditions for screening and experimental desing (33*2). It was found that the best conditions for the enzymatic hydrolysis of cassava starch were: α-amylase (Liquozyme® SC DS) at pH 5.0, temperature at 80 °C, enzyme dosage of 0.0280 % (w/w); and with glucoamylase (Spirizyme® Fuel) at pH 4.5, temperature at 70 ° C, enzyme dosage 0.0631 % (w/w); and finally enzymatic mixture of α-amylasee and glucoamylase (STARGEN 001) at pH 4.5, temperature 46 ° C, and enzyme dosage of 0.023% (w/w).
Keywords: Cassava starch, starch, enzymatic hydrolysis, cassava
PDF file
See more of Poster Session One Sponsored by Genencor
See more of General Submissions
See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)
See more of General Submissions
See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)