Currently, butanol has been considered as a potential liquid biofuel, and the butanol production has long been studied using clostridia strains.  One of the difficulties of using clostridia strains is the formation of mixed-fermentation products, such as butyrate, acetate, acetone and ethanol.  In this study, we engineered Escherichia coli by introducing recombinant butanol pathway from multiple clostridia strains.  Several enzyme coding genes for butanol production were cloned from different clostridium species including Clostridium acetobutylicum ATCC 824, C. beijerinckii ATCC 10132, and C. beijerinckii ATCC 25752.  The recombinant enzymes were expressed under a high constitutive expression (HCE) promoter, and the specific activity and the butanol productivity were compared.  The native E. coli competing pathways, such as lactate, succinate, acetate, and ethanol, were disrupted to enhance the butanol production.  The results showed that using E. coli as a host strain for the production of butanol is promising.