Monday, April 19, 2010
12-40

Characterization of xanthan gum produced starting from sugarcane broth

Sandra Faria1, Carmen Lúcia de Oliveira Petkowicz2, Miriam Maria de Resende1, Sérgio Antônio Lemos Morais3, Manuel Gonzalo Hernandez Terrones3, and Vicelma Luiz Cardoso1. (1) Faculty of Chemical Engineering, Uberlândia Federal University, P.O. Box 593, CEP 38400-902, Uberlândia, Brazil, (2) Centro Politécnico, Universidade Federal do Paraná., Jardim das Américas, Caixa-Postal: 19046, Curitiba, 81531-990, Brazil, (3) Chemical Institute, Uberlândia Federal University, Av. João Naves de Ávila, 2121 - Bloco 1D, Caixa Postal 593, Uberlândia, 38.400-902, Brazil

The balance between the sustainability and productivity of the cane happens through new varieties of adaptable plants to the conditions of each area. In this aspect Brazil is in a privileged position and it is put in the world vanguard of the cane biotechnology. Due to the great offer of this biomass in the Brazil cultivars this work was motivated to the production of xanthan gum by Xanthomonas campestris pv. campestris NRRL B-1459 using diluted sugar cane broth for experiments with duration of 24 h. The gum was produced in the optimized conditions: 27.0 g.L-1 of sucrose; 2.0 g.L-1 of yeast residual brewer; 0.8 g.L-1 NH4NO3; 750 rpm and 0.35 vvm. In this condition the produced gum presented molecular weight and total sugar content, considering the polymerization conducted at the optimum point were 4.172*106 Da and 85.3%, respectively, distributed in 43% glucose, 32% mannose and glucuronic acid 24% in the 1.79:1.33:1 proportion. The infrared spectrum recorded remarkable groups in the elucidation of the compound and, when the samples, commercial and optimized, subject to the comparison, emit your bands in the regions of wavelength equivalent the similarity of the gum has been confirmed. The gum also was evaluated using the Nuclear Magnetic Resonance Proton (1H NMR) and, therefore, identified in the last stage by chemical displacements (ppm) common to the hydrogen nucleus and the presence of α-anomeric protons and protons β carbons of hexoses or pentoses, protons of carbons connected to oxygen, proton H6 of rhamnose and protons of glucuronic and uronic acid.

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