Monday, April 19, 2010
1-26
Modulation of pentose sugar transport by Gal1 in Saccharomyces cerevisiae
In our current study, we looked into the galactose transport to study possible improvements to pentose sugar uptake. Gal2p is a carrier that transports sugars into yeast cells through facilitated diffusion. Another protein in the galactose pathway is galactokinase (Gal1p). We over-expressed Gal2 alone and then Gal1+Gal2 together in a yeast strain that lacked major hexose transporters. Over-expression of Gal2 gene alone or Gal1+Gal2 genes together restores the ability of this strain to grow on glucose and thereby affords us the ability to compare Gal2 alone with Gal1+Gal2. Our results show that strains carrying Gal1+Gal2 have higher xylose and arabinose uptake ability compared to strains carrying Gal2 alone. For comparison we also carried out uptake and fermentation studies of strains over-expressing HXT-7 and GXF1.
See more of General Submissions
See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)