Monday, April 19, 2010
3-48
A method for calculation of enzymatic hydrolysis yield at high solid concentration
Yongming Zhu, Novozymes North America Inc, 77 Perry Chapel Church Road, Franklinton, NC 27525, Marco Malten, Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Bagsvaerd, Denmark, and Mads Torry-Smith, Novozymes North America, Inc., 77 Perry Chapel Church Road, Franklinton, NC 27525.
Calculation of hydrolysis yield at high solid concentration is complicated primarily by two important factors: (1) the change of liquid volume with solid solubilization, and (2) the variation of liquid density due to sugar liberation. When studying the effect of solid concentration on hydrolysis performance, ignoring these factors in yield determination can result in misleading conclusions. In this presentation, we will report a mathematical method developed for hydrolysis yield calculation suitable for the case of high solids loadings. The information needed in the calculation includes the composition of the substrate, initial solid loading, and the sugar concentrations before and after hydrolysis. The method was validated experimentally for solid loadings up to 30 wt% with CV less than 5%. Compared to the other methods found in the literature, this method is easy to use and represents good accuracy.
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)