Monday, April 19, 2010
3-23

Changes on cellulolytic enzyme system during simultaneous saccharification and fermentation

Xu Fang1, Kai Liu1, Lin Xiao2, Yuqi Qin1, Xiaomin Wei1, Guodong Liu1, Mingtian Zhu1, and Yinbo Qu1. (1) State Key Laboratory of Microbial Technology and National Glycoengineering Research Center, Shangdong University, No. 27 Shanda-nan Road, Jinan, 250100, China, (2) Longlive Bio-Technology Co., Ltd., High-technology Development Zone, Yucheng, 251200, China

In a corncob biorefinery process, hemicellulose in corncobs was extracted after pretreated by diluted acid or compressed hot water, and used for the production of xylitol, xylo-oligosaccharide, furfural, and related products. A small portion of corncob residues was utilized for cellulase production, and the crude enzyme preparation was utilized to hydrolyze corncob residues (CCRs). Finally, the hydrolysate was converted into ethanol by yeast.

The structure features of these CCRs pretreated by different methods were analyzed. Simultaneous saccharification and fermentation (SSF) was performed by adding crude cellulase preparations from Penicillium decumbens and yeast (Saccharomyces cerevisiae) at 30oC. It was investigated on ethanol production from CCRs with the method of SSF. Furthermore, the fed-batch method was combined with SSF to enhance ethanol concentration and reduce enzyme dosage. Changes on cellulolytic enzyme system during SSF were observed with SDS-PAGE. The results indicated that lignin removal favored the recovery of CBH I, which is beneficial for the fed-batch method in SSF. Therefore, considerably high ethanol concentration and cellulose conversion from the delignined CCRs could be obtained with low enzyme dosage by combining fed-batch method with SSF.

Acknowledgment This project was sponsored by National High Technology Development Program of China (2006AA020201) and National Key Technology R&D Program of China (2006BAD07A08).


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