Monday, April 19, 2010
3-47

Evaluation of holocellulase production by filamentous fungi grown on agro-industrial residues

Félix Gonçalves de Siqueira1, Aline Gonçalves de Siqueira1, Eliane Gonçalves de Siqueira1, Marly Azevedo Carvalho1, Beatriz Magalhães Pinto Peretti1, Paula Marcela Duque Jaramillo2, Ricardo Sposina Sobral Teixeira3, Eustáquio Souza Dias2, Carlos Roberto Félix1, and Edivaldo Ximenes Ferreira Filho1. (1) Cellular Biology, University of Brasília, Campus Universitário, Brasília, Brazil, (2) Biology, Federal University of Lavras, Lavras, Brazil, (3) Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

Agaricus brasiliensis CS1, Pleurotus ostreatus and Aspergillus flavus produced holocellulase when grown in solid and liquid cultures containing agro-industrial residue, including sugar cane bagasse and dirty cotton residue, as substrates. Those isolates proved to be an efficient producer of holocellulase under the conditions selected for this screening. The bromatological analysis of agro-industrial residues showed some differences, especially for protein, fiber, hemicelluloses, cellulose and lignin contents. The highest holocellulase activities (xylanase, mannanase, polygalacturonase, endoglucanase, FPAse and avicelase) were obtained after solid state cultivation at 10% of substrate concentration. In this case, remarkably levels of xylanase and pectinase activities were produced by A. flavus, followed by P. ostreatus.  A. brasiliensis CS1 showed the best yield of reducing sugar production when grown on medium containing sugar cane bagasse. It was also the most efficient producer of protein, except when cultivated on dirty cotton residue, which had A. flavus as the best producer. The enzymatic hydrolysis of sugar cane bagasse and dirty cotton residue by crude extract samples of A. brasiliensis CS1, P. ostreatus and A. flavus showed that the best yield of reducing sugar production was achieved with sugar cane bagasse as the substrate.
Keywords: Agaricus brasiliensis · Agro-industrial residue · holocellulose · holocellulase · xylanase · pectinase
Supported by CNPq and FAPDF

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