Paper: Kinetics and Synergy of <i>Trichoderma reesei </i>Cellulases on Ionic Liquid Pretreated Avicel and <i>Miscanthus giganticus</i> (31st Symposium on Biotechnology for Fuels and Chemicals (May 3-6, 2009))

Monday, May 4, 2009

InterContinental Ballroom (InterContinental San Francisco Hotel)

Jerome Fox , Chemical Engineering, University of California, Berkeley, Berkeley, CA

Harshal A. Chokhawala , Chemical Engineering, University of California, Berkeley, Berkeley, CA

Craig Dana , Chemical Engineering, University of California, Berkeley, Berkeley, CA

Dana Nadler , Chemical Engineering, University of California, Berkeley, Berkeley, CA

Paul Wolski , Energy Biosciences Institute, Univeristy of California, Berkeley, CA

Harvey W. Blanch , Chemical Engineering, University of California, Berkeley, Berkeley, CA

Douglas S. Clark , Chemical Engineering, University of California, Berkeley, Berkeley, CA

A number of lignocellulose pretreatment methods have been explored to increase the susceptibility of lignocellulose to cellulase action. Certain ionic liquids can dissolve lignocellulose under mild conditions and have been shown to disrupt the hydrogen bonding network of crystalline cellulose. We have compared the activities of purified T. reesei cellulases CBHI, CBHII, and EGI on untreated and ionic-liquid pretreated cellulosic and lignocellulosic substrates. Excess beta-glucosidase was included to reduce potential inhibition by cellobiose. To assess the impact of pretreatment methods on the potential synergistic action of cellulases, the rates of each individual enzyme on pretreated and untreated substrates were compared to those of ternary enzyme mixtures.

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5-59: Kinetics and Synergy of Trichoderma reesei Cellulases on Ionic Liquid Pretreated Avicel and Miscanthus giganticus