3-20: The optimum of Enzymatic hydrolysis from pretreated rice straw by aqueous ammonia

The bio-ethanol has recently received much attention as an alternative energy. Generally the bio-ethanol is produced by fermenting the glucose obtained from biomass. The typical raw materials for bio-ethanol are biomass such as a sugar cane, wheat, corns, potato, barley, and a sweet potato. The bio-ethanol is renewable because raw materials are obtained from plants. Compare to the gasoline discharging pollutants like carbon monoxide, the bio-ethanol is entirely not exhaust hazardous substance. Bioprocesses for converting lignocellulose to useful materials such as liquid fuels and chemicals have been receiving increasing attention. Pretreatment is an essential element in the bioconversion of lignocellulosic substrates. Pretreatment of lignocellulosic biomass using the percolation process was usually determined to be 150°C. Soaking process was carried out mild conditions at atmospheric pressure of 60°C. Compared two pretreatment processes, we found enzymatic hydrolysis condition of pretreated biomass. In case of a rice straw, compared with previous lignocellulosic biomass, we knew that hydrolysis time was short. Also, the optimum conditions for the maximum glucose conversion rate were found to be a temperature, pH, hydrolysis time. Thus by using the control composite design, it is possible to determine the accurate values of the hydrolysis parameters where maximum production of sugar occurs. This study estimates that a rice straw has the possibility of SSF(Simultaneous Saccharification and Fermentation).