Paper: Simultaneous Saccharification and Fermentation of Switchgrass with <i>Kluyveromyces marxianus </i>IMB 3 in pH controlled Bioreactor (31st Symposium on Biotechnology for Fuels and Chemicals (May 3-6, 2009))

Sunday, May 3, 2009

InterContinental Ballroom (InterContinental San Francisco Hotel)

Brian Faga , Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK

Mark R. Wilkins , Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK

Ibrahim M. Banat , School of Biomedical Sciences, University of Ulster, Coleraine, United Kingdom

Previous simultaneous saccharification and fermentation (SSF) of switchgrass experiments using the thermotolerant IMB 3 strain of Kluyveromyces marxianus suggest that maintaining a pH at or slightly above 5.0 will result in increased ethanol production. Switchgrass was pretreated with pressurized hot water at 200 ºC for 10 min. Two 1.5 L SSFs will be performed in a stirred bioreactor. The pH will be automatically controlled at 5.5 and 5.0 by adding 2 M potassium hydroxide and the temperature will be maintained at 45 ºC. 15 FPU/g glucan of cellulase enzyme will be used with a beginning glucan concentration of 40 g/L. Samples will be taken every 24 hours for 7 days and analyzed by HPLC for ethanol, sugars, and organic acid concentrations.

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