Sunday, May 3, 2009
InterContinental Ballroom (InterContinental San Francisco Hotel)
Oligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize CO (carbon monoxide), CO2 (carbon dioxide) or syngas (gas mixture that contains varying amounts of CO and H2 generated by the gasification of organic wastes). Previous reports showed that the megaplasmid pHCG3 encoding the carbon monoxide dehydrogenase/ acetate synthase (CODH/ACS) enzyme complex is involved in the assimilation of CO or syngas. The present study aimed at sequencing and annotation of the circular chromosome to identify pathways responsible for assimilation of carbon that is fixed by chemolithoautotrophy into fatty acids. The knowledge of the complete genome also enabled comparative genomics studies with relatively close bacterial species. Interestingly, fatty acid methyl ester (FAME) analysis of O. carboxidovorans grown in the presence of acetate and syngas showed that the bacterium produces specific fatty acids that are components of biodiesel