Monday, May 4, 2009
InterContinental Ballroom (InterContinental San Francisco Hotel)
Currently ethanol is the most utilised biofuel to supplement conventional gasoline; however recently n-butanol has emerged as a potential alternative fuel additive due to advantages over ethanol such as a higher energy content. 1-butanol is synthesised as a fermentation product by solventogenic Clostridia spp. and genes encoding the butanol pathway in these microorganisms has previously been expressed in Escherichia coli. In order to engineer a novel synthetic butanol pathway in Escherichia coli non-clostridial genes encoding enzymes resulting in the overall synthesis of 1-butanol were expressed from a PBAD promoter. Competing metabolic pathways in the host E. coli were deleted and the effects of these deletions assessed.