9-44: Statistical Optimization of 1,3-propanediol Fermentation Using the Engineered Strain of Klebsiella pneumoniae

1,3-propanediol (1,3-PD) as an important chemical product could be used for synthesis reactions, in particular as a monomer for polycondensations. Klebsiella pneumoniae is one of the several well-known microorganisms, which produce 1,3-propanediol (1,3-PD) from glycerol. K. pneumoniae is an excellent 1,3-PD producer, but too much by-product (2,3-butandiol, Ethanol, Succinic acid, etc.) yielded greatly reduce the fermentation efficiency of 1,3-PD. We developed mutant strains by genetic engineering the glycerol metabolic pathway. In the present study, we attempted to optimize culture conditions for 1,3-PD by the engineered strain of K. pneumoniae using response surface methodology (RSM) based on a 2⁵ factorial central composite design (CCD) where the simultaneous effect of five independent variables, glycerol, aeration volume, pH, cultivation temperature and time.