Monday, May 4, 2009
5-66
Effects of analysis error on the results of enzymatic hydrolysis of acid pretreated bagasse
Lab scale enzymatic hydrolysis of cellulosic biomass is usually carried out at predetermined percentage of substrates loading and dosage of enzymes. For the saccharification of acid pretreated sugarcane bagasse (APB), glucan content and moisture content of APB are the two key factors that influence the amount of substrate loading, while total protein content of cellulase, if enzyme is dosed on total protein, is the factor that affects the amount of enzyme used in a particular experiment. It is obvious that saccharification results will be influenced by the analysis error of the aforementioned three key factors. In this poster, we will present the results of a Design of Experiment we conducted, and demonstrate how different the APB saccharification results will be if a small error (i.e., 5%) in glucan, moisture, or total protein content analysis, is introduced.
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