Sunday, May 3, 2009
3-46
Profiling of Oligosaccharides and Related Degradation Products Generated by AFEX Pretreatment of Lignocellulosic Biomass using LC/TOF-MS
This study presents methods for quantitative profiling of oligosaccharides and related degradation products following Ammonia Fiber Explosion (AFEX) pretreatment of corn stover using different thermochemical severity conditions. Solid phase extraction conditions were optimized for enrichment of oligosaccharides using porous graphitized carbon, and LC/TOF MS using electrospray ionization with multiplexed collision induced dissociation (CID) was used to analyze the degradation products. The study revealed presence of oligosaccharides up to degree of polymerization (DP) 22, with and without various chemical modifications including acetylation. Multivariate statistical analyses highlighted variations in oligosaccharide content among untreated and different severity AFEX-treated corn stover. Chemical characterization of AFEX byproducts reveals the extent of oligosaccharide modification using different pretreatment conditions, and provides valuable information for process optimization.