Sunday, May 3, 2009
2-20
The effect of different nitrogen and carbon sources on biomass of local isolates of Clostridium acetobutylicum
Ausama. M. S. Al-Naemi Ausama. M. S. Al-Naemi, Biology, Mosul University, Iraq, Mosul, Hi AL-Refai, Hi Al-refai, Mosul, 964, Iraq
ABSTRACT Three local isolates of C. acetobutylicum obtained from untreated wastewater
and sludge were exposed to microwave radiation for different lengths of time and
at different distances from the radiation source. To investigate the possible
improvement in biomass production created in these strains, they were then grown
in media containing different combinations of carbon and nitrogen sources, and
compared to a control of C. acetobutylicum originally obtained from ATCC. The roles of lithium, potassium, and sodium were also investigated. Base media contained corn grits, calcium carbonate, ammonium sulfate, and sodium phosphate. The highest biomass obtained as measured by dry weight was 18.6 g/L (control strain with di-ammonium hydrogen orthophosphate and lithium bicarbonate) and the lowest was 0.02 g/L (control strain with ammonium
bicarbonate as both carbon and nitrogen source).
and sludge were exposed to microwave radiation for different lengths of time and
at different distances from the radiation source. To investigate the possible
improvement in biomass production created in these strains, they were then grown
in media containing different combinations of carbon and nitrogen sources, and
compared to a control of C. acetobutylicum originally obtained from ATCC. The roles of lithium, potassium, and sodium were also investigated. Base media contained corn grits, calcium carbonate, ammonium sulfate, and sodium phosphate. The highest biomass obtained as measured by dry weight was 18.6 g/L (control strain with di-ammonium hydrogen orthophosphate and lithium bicarbonate) and the lowest was 0.02 g/L (control strain with ammonium
bicarbonate as both carbon and nitrogen source).