Sunday, May 3, 2009
3-39
SACCHARIFICATION OF IONIC LIQUID PRETREATED BIOMASS WITH DIFFERENT ENZYME MIXES
Indira P. Samayam1, Amy Coxe1, Jacob Wheeler1, Anantharam P. Dadi2, Constance A. Schall2, and Sasidhar Varanasi1. (1) Chemical and Environmental Engineering, UNIVERSITY OF TOLEDO, 2706 KENDALE DR APT#204, Toledo, OH 43606, (2) Department of Chemical & Environmental Engineering, The University of Toledo, 2801 W Bancroft street, W Central Ave, Apt # C10, Toledo, OH 43606
The ionic liquid pretreatment of lignocellulosic biomass produces amorphous or partially crystalline cellulose and enhances the saccharification rates of biomass sugars. The high crystallinity of cellulose poses an impediment to hydrolysis enzymes for the cellulosic portion of biomass. Different pretreatment conditions have been explored to produce amorphous cellulose to increase accessible surfaces to the enzymes used in saccharification. Variable enzyme mixes have been used to study the saccharification kinetics in an attempt to explore a simplified enzyme component mixes and to reduce the overall cost of the process. Cellulose and biomass pretreated with ionic liquid have been characterized to assess the crystallinity and elucidate the acessibility of cellulase enzymes to biomass. Saccharification results are compared for pretreated biomass of variable crystallinity.