Compositional carbohydrate analysis of new biocrop feedstocks is essential to the efficient production of cellulosic ethanol.  The analysis is commonly done using HPLC with refractive index (RI) detection, however this method has several limitations.  HPLC-RI system robustness can be severely limited by the variation in RI detector response due to changes in temperature, and reversed-phase separations often require long run times and lack sufficient resolution to detect biomass sugars.  While high-performance anion-exchange with pulsed amperometric detection (HPAE-PAD) technology permits baseline-resolved separation and detection of biomass sugars in complex samples, its high sensitivity requires dilution prior to analysis, which often introduces error into the measurement.  In this poster we present a rapid method (<10 min) for monosaccharide analysis, and determination of composition, based on HPAE-PAD that enables direct injection of corn stover hydrolysates with no dilution.  We present our results for performance, including precision (0.2-0.3% retention time and 0.7-1.3% peak area RSDs), accuracy (85-96% spike recovery), limits of detection (approximately 0.002 ppm), and linearity (r2 = 0.9997).