Simultaneous saccharification and fermentation would vastly improve the prospects of large-scale economical production of Lignocellulosic ethanol. One of the approaches to achieve this objective is to use a whole cell biocatalyst that can express clostridium cellulose enzyme system on the surface of ethnogenic Escherichia coli LY01. LY01 is capable of co-metabolizing pentoses and hexoses. This approach has been successfully demonstrated in our work with amorphous cellulose and dilute acid pretreated Corn Stover. However, scale-up of such a system would require an understanding of transport phenomena in the heterogeneous hydrolysis of cellulose by such whole cell biocatalyst. In particular, we consider the hydrolysis of dilute acid pretreated Corn Stover by this system in a bioreactor. A model is developed that can account for pore sizes, particle sizes and flow characteristics. The validity of this model will be tested against fermentation data from a bioreactor. The bioreactor allows the control of conditions especially pH which critically affects the enzyme activity. The effect of rate of agitation and particle size distribution are of particular interest. Experiments have also been performed to demonstrate co-metabolism of hexoses and pentoses, which would reduce the entire process to one-step ethanol production