Monday, May 4, 2009
5-59
Kinetics and Synergy of Trichoderma reesei Cellulases on Ionic Liquid Pretreated Avicel and Miscanthus giganticus
Jerome Fox1, Harshal A. Chokhawala1, Craig Dana1, Dana Nadler1, Paul Wolski2, Harvey W. Blanch1, and Douglas S. Clark1. (1) Chemical Engineering, University of California, Berkeley, Calvin Hall Rm 250, University of California, Berkeley, CA, 94720, Berkeley, CA 94720, (2) Energy Biosciences Institute, Univeristy of California, Berkeley, CA 94720
A number of lignocellulose pretreatment methods have been explored to increase the susceptibility of lignocellulose to cellulase action. Certain ionic liquids can dissolve lignocellulose under mild conditions and have been shown to disrupt the hydrogen bonding network of crystalline cellulose. We have compared the activities of purified T. reesei cellulases CBHI, CBHII, and EGI on untreated and ionic-liquid pretreated cellulosic and lignocellulosic substrates. Excess beta-glucosidase was included to reduce potential inhibition by cellobiose. To assess the impact of pretreatment methods on the potential synergistic action of cellulases, the rates of each individual enzyme on pretreated and untreated substrates were compared to those of ternary enzyme mixtures.