Monday, May 4, 2009
9-57
MEDIUM COMPOSITION EFFECT ON THE EXPRESSION OF LACK ANTIGEN OF Leishmania chagasi
Michelle Rossana Vaz Sr., Sirtys Santos Andrade, Daniela Regina Martins, Everaldo Silvino Santos, and Gorete Ribeiro Macedo. Engenharia Química, Universidade Federal do Rio Grande do Norte, Campus Salgado Filho Lagoa Nova, Natal, Brazil
With the advent of the recombinant DNA technology, the recombinant protein expression becomes an important path in the studies of the structure, function and identification of new proteins, especially ones with therapeutic objectives. Escherichia Coli has been the predominant prokaryotic used in the genetic engineering studies due to the abundance of information about its metabolism. Despite the expressive advance of the studies about the molecular biology and immunology of the infections, there is not, actually, any prophylactic drug capable to prevent the kalazar. Theferore, there is a real necessity for identifying specific antigens for the development of vaccines and diagnostic kits against the Visceral leishmaniasis. In this context, this work aimed to study the expression of the LACK recombinant antigen of the Leishmania chagasi during the Escherichia coli cultivation in shaker. In a set of assays it was carried out an induction procedure using IPTG, in order to observe the influence of the media composition (2xTY, TB) under expression of the LACK recombinant protein. Results showed that LACK recombinant clone expression was able to express the interest protein (LACK), confirmed by electrophoresis. On the other hand, it was observed the high complexity of the TB cultivation medium was limiting in the protein production.