Monday, May 4, 2009
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Characterization of the specific activities and hydrolytic properties of the cell-wall degrading enzymes produced by Trichoderma reesei RUT C30 on different carbon sources
The hydrolytic properties of the enzymes were compared with the commercial Celluclast 1.5L on four substrates: SPCS, steam pretreated spruce (SPS), SF and Avicel using a standard FPU loading. Enzymes produced on the lignocellulosic substrates performed generally better than the commercial one. As expected, the substrates containing xylan were clearly hydrolyzed more efficiently with enzymes rich in xylanases, whereas the cellulosic substrates depended more on the cellulase enzyme pattern. Hydrolysis of SPCS and SPS was also performed with various enzyme preparations (Celluclast and the enzymes produced on SPCS and SF) by adjusting with purified enzyme components the xylanase and ß-glucosidase levels to reach similar activity levels. The results lead to further understanding of the hydrolytic mechanisms and the factors determining the performance of different mixtures of T. reesei enzymes.
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