The determination of oil content in biodiesel feed stocks can be performed using several methods, including mechanical press, solvent extraction, and Nuclear Magnetic Resonance. For the feedstock quality control in terms of oil content, it is important that the applied method is universally accepted so as to obtained results that can be compared with those reported from alternate sources. Although the EN (european norm) has specified two methods for the determination of oil content in oil seed crops, i.e. a conventional Soxhlet extraction and NMR imaging, they also have some disadvantages, including time-consuming, intensive labour input, requiring of highly skill labor, significant amount of sample, high cost, as well as being unfriendly to the environment. The accelerated solvent extraction technique developed by Dionex (ASE-100) has great potential to overcome these constraints.  Furthermore, it also has a high possibility to be applied with the oil content testing of the third generation of biodiesel feedstocks, i.e. microalgae. In this  paper we present the extractraction of oil from Jatropha seeds in compliance with accuracy and reproducibility requirements described in the European standard method. Oil extraction using ASE-100 requires only one to one and a half hours as compared to nine hours consumed by the Soxhlet extraction. In this research, the effects of ASE-100 conditions on the percentage of oil extracted, the optimum conditions for oilseeds extraction and the minimum amount of oilseeds required per test are also investigated.