Monday, May 4, 2009
9-16
Optimisation of Lipase Production by Citrobacter freundii
Ankush Innani, Depatment of Bioptechnoligy, University of Newcastle,, 24 Dilston Road, Fenham, Newcastle upon tyne – NE4 5AA, UK, Newcastle upon tyne, United Kingdom
Lipases being hydrolases occupy a prominent place among biocatalysts and have a wide array of Biotechnological applications.In the current research the production of Lipase from Citrobacter freundii is canvassed. Citrobacter freundii was isolated and identified from sewage sample and it was screened for the production of lipase. The organism was inoculated for the production of lipase in the production medium. After the production the broth was subjected to downstream processing and the activity (2, 00,000 U/mg) was computed using precipitation, dialysis & ion exchange chromatography. The enzyme showed optimum activity at pH 5.0, Temp 37°C and with 0.2 mg/ml Activator MgCl2.