White rot fungi efficiently degrade lignin and provide an excellent starting point for deriving biologically inspired strategies for industrial-scale lignin degradation. The breakdown of lignin involves both enzymes and small molecule mediators and cofactors. In this study we explore how the set of small molecules secreted by P. Chrysosporium and released from degraded wood (the degradome) changes as a function of colonization time. P. Chrysosporium was cultivated on a substrate of poplar wafers, initially enriched with potato dextrose broth. The degradome was analyzed using LC-FTMS, following acetone extraction of the wafers. Wafers were harvested after zero, one, two, three and four weeks of culturing, and mass spectrometric profiles were compared to assess changes in the degradome as a function of colonization time. We report our progress in analysis of these profiles and identification of small molecules correlated with lignin degradation.