Monday, May 4, 2009
9-44

Statistical Optimization of 1,3-propanediol Fermentation Using the Engineered Strain of Klebsiella pneumoniae

Baek-rock Oh1, Mi-Young Seo2, Jeong-Woo Seo2, Chul Ho Kim2, and Don-Hee Park3. (1) Molecular Bioprocess Research Center, KRIBB; Interdisciplinary Program of Graduate School for Bioenergy & Biomaterials, Chonnam National University, Gwangju, South Korea, (2) Molecular Bioprocess Research Center, KRIBB, Jeonbuk, 580-185, South Korea, (3) Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, School of Biological Sciences and Technology, Chonnam National University, Gwangju, South Korea

1,3-propanediol (1,3-PD) as an important chemical product could be used for synthesis reactions, in particular as a monomer for polycondensations. Klebsiella pneumoniae is one of the several well-known microorganisms, which produce 1,3-propanediol (1,3-PD) from glycerol. K. pneumoniae is an excellent 1,3-PD producer, but too much by-product (2,3-butandiol, Ethanol, Succinic acid, etc.) yielded greatly reduce the fermentation efficiency of 1,3-PD. We developed mutant strains by genetic engineering the glycerol metabolic pathway. In the present study, we attempted to optimize culture conditions for 1,3-PD by the engineered strain of K. pneumoniae using response surface methodology (RSM) based on a 25 factorial central composite design (CCD) where the simultaneous effect of five independent variables, glycerol, aeration volume, pH, cultivation temperature and time.

See more of Poster Session 2/Reception
See more of The 31st Symposium on Biotechnology for Fuels and Chemicals (May 3-6, 2009)