Monday, May 4, 2009
11-28
Influence of nutrients on C50-carotenoids production by Haloferax mediterranei ATCC 33500
This study was to investigate the cultural conditions for the production of C50-carotenoids by Haloferax mediterranei ATCC 33500 using a two-stage strategy. At the 1st stage culture, ATCC 1176 medium was used as the basal medium and studied on increasing the growth of H. mediterranei ATCC 33500 biomass. The 2nd stage culture was simulated as resting cells culture of the 1st stage harvested cells to conduct the bioconversion of C50-carotenoids and studied on the influence of nutrient factors and cultural conditions for C50-carotenoids production at the stage. The results indicated that the optimum conditions for C50-carotenoids production by H. mediterranei ATCC 33500 were to use ATCC 1176 medium contained 1% glucose as the initial medium and incubated the cells to the mid-log phase of growth under 37°C , 150 rpm of agitation and 1 vvm of aeration at the 1st stage culture. Successively, the 2nd stage was proceed to the bioconversion of C50-carotenoids by means of incubating the 1st stage harvested cells in the synthetic salts medium (briefly contained at 5% NaCl, 0.1% CH3COONa and 8% MgSO4.7H2O) under 37°C ,120 rpm of agitation for 24 hr. The yield of C50-carotenoids was reached 0.604±0.005 Abs/mL broth.
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