Two specific enzyme blends (EB1 & EB2) were prepared in the laboratory from commercial enzyme sources and tested on 45 different combinations of cellulosic fibers in simultaneous saccharification and fermentation (SSF) experiments. EB1 contained the starch degrading enzymes glucoamylase and α-amylase as well as cellulase and hemicellulase activities while EB2 was formulated without glucoamylase or α-amylase.  The fibers evaluated included chemically treated and untreated blends of corn fiber, wheat chaff, wheat straw, corn stover, switchgrass, and/or soluble distillers dried grains (DDGS). The ethanologens evaluated were both Saccharomyces cerevisiae strains:  r424A (Purdue University,) selected for its ability to utilize xylose, and Y500 (ADM,) selected for its ability to withstand intense inhibitory factors. The highest ethanol concentration and yield was observed using 424A in combination with EB1. Due to their higher residual starch content, corn fiber/DDGs blends combined with EB1 generated the highest production of ethanol for both strains of yeast.