Monday, May 5, 2008
7-36
Changes on performance and microbial population induced by hydraulic retention time in a pilot plant thermophilic anaerobic digester
Teodoro Espinosa-Solares1, David H. Huber2, Ami M. Smith2, John Bombardiere3, Max Domaschko4, and Mark Chatfield2. (1) Ingeniería Agroindustrial, Universidad Autónoma Chapingo, Apartado Postal # 161, Chapingo, Edo de México, Mexico, (2) Department of Biology, West Virginia State University, 101 Hamblin Hall, Institute, WV 25112-1000, (3) Consultant for Enviro Control Ltd., Singleton Court Business Park, Wonastow Road, Monmouth, United Kingdom, (4) Gus R. Douglass Land-Grant Institute, West Virginia State University, 201 ACEOP Administration Building, Institute, WV 25112-1000
Bacterial and archaeal communities, as well as the digester performances, were analyzed in order to evaluate the influence of hydraulic retention times. A 40m3 pilot plant anaerobic thermophilic bioreactor was used for the experiments and fed with poultry litter. Chemical composition of feed, effluent and biogas were monitored throughout the experiment. Mass and energy balances were used to evaluate the performance of the biodigester. Samples of
microbial community DNA were collected each week during the experiment. The relative abundances of dominant microbial populations were measured by terminal restriction fragment length polymorphism (T-RFLP) using PCR primers targeting the bacteria and archaea. Results indicate that loading rate plays an important role in biogas production and reduction of COD and BOD. These changes were associated with variable microbial population dynamics in the bioreactor. T-RFLP profiles of the bacteria indicate that 4 ribotypes dominated the profile throughout the experiment. The abundance of 2 of the 4 ribotypes changed during the course of the experiment while 2 ribotypes remained relatively constant. Archaeal T-RFLP profiles revealed 1 dominant ribotype which does not appear to change in abundance. The results of this experiment, both power input and biodigester performance, indicate that the 13d HRT was optimal for the anaerobic digestion of poultry litter in the experimental conditions evaluated here.
microbial community DNA were collected each week during the experiment. The relative abundances of dominant microbial populations were measured by terminal restriction fragment length polymorphism (T-RFLP) using PCR primers targeting the bacteria and archaea. Results indicate that loading rate plays an important role in biogas production and reduction of COD and BOD. These changes were associated with variable microbial population dynamics in the bioreactor. T-RFLP profiles of the bacteria indicate that 4 ribotypes dominated the profile throughout the experiment. The abundance of 2 of the 4 ribotypes changed during the course of the experiment while 2 ribotypes remained relatively constant. Archaeal T-RFLP profiles revealed 1 dominant ribotype which does not appear to change in abundance. The results of this experiment, both power input and biodigester performance, indicate that the 13d HRT was optimal for the anaerobic digestion of poultry litter in the experimental conditions evaluated here.
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See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)
See more of General Submissions
See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)