Monday, May 5, 2008
6-24

Effects of xylanases addition on enzymatic hydrolysis of biomass

Chuanbin Liu, Christina Gutierrez, Tom Huang, and Colin Mitchinson. Genencor, A Danisco Division, 925 Page Mill Road, Palo Alto, CA 94304

Hemicellulose is a major component of lignocellulosic biomass. The content of xylan left in the pretreated biomass varies dramatically, depending on the source of the biomass, the pretreatment method used, and the severity of pretreatment. For instance, dilute acid pretreated corn-stover contains typically 3% of xylan in the washed solids, while steam expanded corn-stover has about 10% xylan. From an economic perspective, the hydrolysis of xylan, and converting the sugars contained in the hemicellulose fraction of pretreated biomass to ethanol is critical to biorefinery. As the structure of xylan is variable, including not only linear β-1,4-linked xylose chains, but also highly branched heteropolysaccharides, the hydrolysis of xylan requires the synergistic action of many enzymes. The effects of adding a range of Genencor xylanases on the enzymatic hydrolysis of the xylan, and glucan, of differently-pretreated lignocellulosic biomass will be reported. 

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