Monday, May 5, 2008
9-08

Effect of BSA treatment of cellulosic biomass on continuous enzymatic hydrolysis and SSF

Simone Brethauer1, Michael Studer2, Bin Yang2, and Charles Wyman2. (1) Center for Environmental Research and Technology and Chemical and Environmental Engineering Department, University of California, Riverside, 1084 Columbia Avenue, Riverside, CA 92521, (2) Center for Environmental Research and Technology, Bourns College of Engineering, University of California, Riverside, 1084 Columbia Avenue, Riverside, CA 92521

The addition of BSA as a model non-catalytic protein has been shown to enhance cellulose hydrolysis rate and reduce the amount of enzyme needed to reach a certain conversion. Furthermore, continuous processes are generally favored for producing fuels such as ethanol as well as commodity chemicals, but data on continuous biological processing of lignocellulosic biomass are scarce. Therefore, the effect of BSA addition on hydrolysis performance in batch as well as continuous experiments is examined. Insights gained after optimizing BSA supplementation for batch hydrolysis and SSF experiments with acid pretreated corn stover and Avicel with respect to the addition mode and the optimal BSA concentration are applied to continuous hydrolysis and SSF of both substrates.

The effects of different operating procedures on sugar and ethanol yields and the possible mechanisms underlying the enhancement in performance by BSA addition will be examined and discussed.


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