Monday, May 5, 2008
6-42

Sugar cane bagasse enzymatic hydrolysis by Trichoderma reesei cellulases

Ayla Santana Silva1, Felipe Moura Knopp1, Leda M. F. Gottschalk1, Clarissa C. Perrone2, Guido Zacchi3, and Elba Bon1. (1) Biochemistry - Chemistry Institute, Federal University of Rio de Janeiro, Instituto de Quimica, sala 539 - Cidade Universitaria, Ilha do Fundao, Rio de Janeiro, Brazil, (2) Organic Chemistry, National Institute of Technology, Avenida Venezuela, 82 - sala 514, Centro 20081-312, Rio de Janeiro, Brazil, (3) Chemical Engineering, Lund University, P.O.Box 124, SE- 22100, Lund, Sweden

Four billion gallons of alcohol fuel were produced in Brazil, in 2006, from a total sugar cane production of 427 million tons. The residue (crushed cane stalk – bagasse) formed after the extraction of the sucrose juice amounts to 75 million tons of dry biomass annually produced. Although each sugar and/or alcohol mill burns the bagasse for the production of steam (heat) and for power/electricity generation, there is a 12% surplus available for the production of biomass ethanol, that increases fuel production per planted area.  In this study, Trichodema reesei RUT C30 was cultivated in lactose, wheat bran and steam treated bagasse (STB) (used in Brazil as cow feed) for the production of cellulolytic enzymes and beta-glucosidase. The performance of the crude enzyme preparations were evaluated for the hydrolysis of “STB” and “STBL”, a steam treated bagasse at Lund University in presence of an acid catalizer. For comparison, the performance of the commercial enzymes GC220 and Spezyme CP (both from GENENCOR) were also evaluated. In all cases, reaction medium for the enzymatic hydrolysis contained 25 g/L bagasse and 10 FPU/g dry biomass. Hydrolyses were performed at 50 ºC, pH 5.0 and 48 hours. The hydrolysis of “STB”, for all enzyme preparations, resulted on 7.8 g/L glucose excepted for GC 220 that resulted on 6.5 g/L. The hydrolysis of “STBL” using Spezyme CP and the other enzyme preparations resulted approximately on 14 g/L and 12.4 g/L glucose respectively, showing that “STBL” is more accessible for the attack of Trichoderma cellulases.

See more of Poster Session Two
See more of General Submissions

See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)