Monday, May 5, 2008
6-36

Cellulases production by Aspergillus fumigatus FBSPE-05 isolated from sugar cane bagasse

Andre Luis Grigorevski-Lima1, Aline Simões da Rocha Bispo2, Danilo Tosta Souza2, Fábio Nuno Marques Da Vinha1, Elba Pinto da Silva Bon3, Rosalie Reed Rodrigues Coelho1, and Rodrigo Pires do Nascimento2. (1) Departamento de Microbiologia Geral, Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompowsky, s/n - Bloco I - Centro de Ciências da Saúde, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, 21941590, Brazil, (2) Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Rua Rui Barbosa, s/n - Centro, Cruz das Almas, 44380-000, Brazil, (3) Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Cidade Universitária CT, Bloco A, Ilha do Fundão, Rio de Janeiro, Brazil

Aspergillus fumigatus, one of the wide range cellulase-producing organisms available, is ubiquitous in Brazil, being found in soil, as well as in agro-industrial residues. In our studies aiming at isolating cellulolytic fungi from sugar cane bagasse, a promising strain was selected and identified as Aspergillus fumigatus FBSPE-05. This strain was tested for cellulases production in submerged fermentation in a mineral medium using two main carbon sources, sugar cane bagasse and wheat bran, and corn steep liquor as nitrogen source. Sugarcane bagasse was the best substrate for CMCase production in these conditions, giving values of 360 U/L after six days fermentation, at 30oC and pH 4.8. For FPase production both carbon sources were good substrates, giving values around 46U/L after two or three  days fermentation in the same conditions.  Solid state fermentation was also tested, using brewer’s spent grain, sugar cane bagasse and wheat bran as carbon sources and corn steep liquor and sodium nitrate as nitrogen source. After enzyme extraction, the highest level of extracellular CMCase (21.06 U/g substrate) was obtained in 1:2 solid:liquid ratio, sugar cane bagasse as carbon source and corn steep liquor as nitrogen source, at 30ºC after 4 days fermentation. The partial characterisation of enzyme activity have shown the best activity at 65ºC and pH 2.0. More studies will be carried out to better characterize cellulase activity by Aspergillus fumigatus, but our previous results indicate that this organism produces a thermophilic acidic-cellulase.

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