Large amounts of animal manure from livestock industry have created great environmental concerns during the past decade. These manures can, however, be a potential resource for producing value-added bioproducts. In this study, dairy manure was used as sole substrate for ligninase production by the white-rot fungus Phanerochaete chrysosporium. Upon the characterization, raw manure contained a variety of nutrients including undigested crude fiber (12% of lignin, 11% of hemicellulase, 27% of cellulose), protein (18%), and notable amounts of minerals. Batch experiments were carried out in shaking flasks with P. chrysosporium pellets for ten days at 37ºC without addition of veratryl alcohol as inducer. Effect of agitation (50-200 rpm) and manure concentration (5-20 g/L, dry basis) on the ligninase production were investigated respectively. The popular lignin-degrading enzyme, manganese peroxidase (MnP) production was found the highest at 100 rpm and 15 g/L manure. After 96 h incubation, the manure culture had a maximum MnP activity of 481 U/L. The result was further visualized with the total excreted proteins on Sodium Do-decylsulfate-Polyacrylamide Gel Electrophesis (SDS-PAGE), suggesting that dairy manure is a suitable raw material for the production of ligninases and can be utilized for the mass production of the enzyme for the biotechnological applications. Future study is warranted to investigate the effect of pH, temperature, etc., to optimize the enzyme production system for continuous process. The expression analysis of total excreted proteins in the dairy manure should also be investigated to provide useful information for the identification of the associated proteins with ligninolytic enzymes.