The yeast species Saccharomyces cerevisiae has been used for millennia to convert sugars to ethanol. Over 1,000 other yeast species are known, though only a limited number are used for production of platform chemicals. We have examined a series of non-Saccharomyces species for production of commercially important compounds including potential biofuels. The Phaff Yeast Culture Collection at the University of California Davis contains thousands of yeast strains isolated from a variety of degrading plant materials. In a preliminary screening study, we have identified and quantified volatile compounds produced by 25 different species. Samples of volatiles in the headspace above duplicate 4-day liquid cultures grown in standard lab media were extracted using Solid Phase Microextraction (SPME) with DVB/CAR/PDMS (divinylbenzene/carboxen/polydimethylsiloxane) fibers followed by GC-MS analysis. Numerous compounds were identified in each sample by comparison with the retention time and mass spectrum of authentic standards. Selected compounds were quantified, including isoamyl alcohol, isobutanol, isobutyl acetate, phenethyl acetate, and phenethyl alcohol. Each yeast species produced a unique and predictable combination of volatile compounds, including alcohols, esters, aldehydes, and ketones. While isoamyl alcohol was produced by most cultures at trace levels, one yeast species produced over 0.5% (w/v). Isobutanol was also detected in trace quantities in the headspace above all cultures, but was produced at a level of over 0.5% (w/v) by two yeast species. Future plans include screening a large number of yeast species to identify those that produce the highest levels of potential biofuel compounds such as higher alcohols and esters.