A wide range of biomass substrates and pretreatments have been evaluated for enzymatic hydrolysis performance.  Presented here are approximately 80 material samples analyzed with Genencor’s first commercially available biomass cellulase, Accellerase™ 1000.  These are represented by pretreatments such as dilute acid, steam expansion, autocatalysis, and two-stage acid/alkali on substrates such as corn stover, sugar cane bagasse, wood pulp, rice straw, and corn fiber.  Saccharifications were performed in 100mL reaction volumes in shake-flasks. Each substrate has been surveyed at a loading of 1%, 7%, and, when possible, 13% cellulose.  Enzyme loadings were fixed at 20 and 80mg total protein/g cellulose.  Substrates were then ranked on “ease of saccharification” based on a benchmark criterion of what dose of Accellerase™ 1000 would be required to reach 80% glucan conversion in 3 days at a 7% cellulose load. Substrate attributes and pretreatment conditions were correlated with hydrolysis performance such as to give further insight into what makes biomass most amenable to enzymatic hydrolysis.