Monday, May 5, 2008
6-32
Selecting a Cellulase for Biomass
Bradley Kelemen and Suzanne Lantz. Genencor International, 925 Page Mill Road, Palo Alto, CA 94304
Not all cellulase mixtures are created the same. This is true for cellulases from different organisms, but also for cellulases produced by the same organism under different conditions. Genencor produces cellulases for a number of industries, with performance criteria set accordingly. To evaluate the range of biomass conversion performance that could be expected from T. reesei-produced cellulases, we collected 61 independent cellulase samples. Whole cellulase (from seven T. reesei strains, three production processes, 1996-2006 production lots of both commercial and experimental samples, and several product formulations) were used to convert 4 cellulosic substrates to sugars. The results will show a significant range of cellulose conversion efficiency and that no one production parameter is solely responsible for generating a cellulase with good or poor biomass performance. The results show that repeated production of cellulase under the same controlled conditions produces a cellulase with predictable and reproducible performance. No one substrate was predictive of the others but good (or poor) conversion of one substrate did correlate with good (or poor) conversion of another substrate. These results emphasize the importance of optimizing and controlling cellulase production specifically for the biomass industry. To that end, Genencor developed and commercialized the first cellulase product specifically for biomass conversion.
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See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)
See more of General Submissions
See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)